Optimizing Recombinant Protein Production in the Escherichia coli Periplasm Alleviates Stress

Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli

Objective(s): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. IgY technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. The purpose of this study was to produce specific antibody by egg yolk antibody (IgY...

Advances and challenges in recombinant protein expression in Escherichia coli

Recombinant Proteins in Escherichia coli: Optimizing Production Strategies

The recombinant proteins are under special attention due to their medical, pharmaceutical and industrial uses. Bacteria are simple and cost effective hosts for producing recombinant proteins. The Gram-negative bacterium Escherichia coli is a high yielding recombinant protein production system, due to its specific characteristics such as the rapid and high-density growth, need of poor medium and...

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

BACKGROUND In Escherichia coli many heterologous proteins are produced in the periplasm. To direct these proteins to the periplasm, they are equipped with an N-terminal signal sequence so that they can traverse the cytoplasmic membrane via the protein-conducting Sec-translocon. For poorly understood reasons, the production of heterologous secretory proteins is often toxic to the cell thereby li...

Strategies for the production of recombinant protein in Escherichia coli.

In the recent past years, a large number of proteins have been expressed in Escherichia coli with high productivity due to rapid development of genetic engineering technologies. There are many hosts used for the production of recombinant protein but the preferred choice is E. coli due to its easier culture, short life cycle, well-known genetics, and easy genetic manipulation. We often face a pr...